Development of a Porcine Slaughterhouse Kidney Perfusion Model

Machine perfusion techniques are becoming standard care in the clinical donation and transplantation setting. However, more research is needed to understand mechanisms of protective effects machine perfusion. For preservation related experiments, porcine kidneys acceptable alternatives human kidneys, because their size similar physiology. In this experiment, use slaughterhouse was evaluated with normothermic kidney (NKP), thereby avoiding laboratory animals. Porcine were derived from two local abattoirs. To induce different degrees injury, warm ischemic times used. After preservation, reperfused for 4 h NKP solutions test renal function damage. The effect technique or a short time clearly seen functional markers, such as creatinine clearance fractional sodium excretion levels, well generic damage marker lactate dehydrogenase (LDH). useful alternative animals transplantation- preservation-related questions. maintain during NKP, hypothermic phase mandatory.